Congratulations to Kashish for a publication in Nature Protocols

July, 2022

Congratulations to Shiying for a publication in Journal of Cell Science

June, 2022

Warm Welcome to Valeria Ortiz who's joining us for the Summer as MUST intern

June, 2022

Zhong Xueying gave a talk at the IUPESM 2022 conference

June, 2022

Tony & Zhong Xueying gave talks at World Congress in Biomechanics 2022 

July, 2022

2021-

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• Kashish Jain, Pakorn Kanchanawong, Michael P. Sheetz, Haogang Cai, Rishita Chengede. “Ligand functionalization of titanium nanopattern enables the analysis of cell–ligand interactions by super-resolution microscopy” Nature Protocols 2022, 1-33. doi: 10.1038/s41596-022-00717-3

• Shiying Liu, Pakorn Kanchanawong, “Emerging Interplay of Cytoarchitecture, Cytomechanics, and Pluripotency Regulation” Journal of Cell Science (135, no. 12 (2022): jcs259379 doi.org/10.1242/jcs.259379

• Shiying Liu, Zhen Zhang, Pakorn Kanchanawong. “Meshworks Analyzer: Quantitative analysis software for super-resolved actin cortex architecture” Software Impacts doi.org:10.1016/j.simpa.2021.100153

• M. Joy-Immediato, R. M. Ramirez, M. Cerda, Y. Toyama, A. Ravasio, P. Kanchanawong*, C. Bertocchi*. “ER structural organization affects mechanotransduction at cadherin-based adhesions by modulating PTP1B activity.” Frontiers in Cell and Developmental Biology doi: 10.3389/fcell.2021.669086 *: co-corresponding authors

• Azita Gorji, Pearlyn Jia Ying Toh, Hui Ting Ong, Yi-Chin Toh, Yusuke Toyama, Pakorn Kanchanawong. “The enhancement of endothelialization by topographical features is mediated by PTP1B-dependent endothelial adherens junctions remodeling.” ACS Biomaterials Science and Engineering doi:10.1021/acsbiomaterials.1c00251

2011-20

• Miao Yu, Shimin Le, Samuel Barnett, Zhenhuan Guo, Xueying Zhong, Pakorn Kanchanawong* and Jie Yan* “Implementing Optogenetic Modulation in Mechanotransduction.”  Physical Review X 10 (2), 021001 doi.org/10.1103/PhysRevX.10.021001 *: corresponding authors

• D. Thumkeo, Y. Katsura, P. Beemiller, Y. Nishimura, P. Kanchanawong, T. Ishizaki, S. Kitajima, C. Takahashi, T. Hirata,  N. Watanabe, M. F. Krummel, S. Narumiya. “mDia1/3-dependent actin polymerization spatiotemporally controls LAT phosphorylation by Zap70 at the immune synapse” Science Advances. 6(1):eeay2432, 2020 doi: 10.1126/sciadv.aay2432

• Yinan Wang#, Samuel Barnett#, Shimin Le, Zhenhuan Guo, Xueying Zhong, Pakorn Kanchanawong*, Jie Yan*. “Label-free single-molecule quantification of rapamycin-induced FKBP-FRB dimerization for Direct Control of Cellular Mechanotransduction” Nano Letters 19(10) 7514-7525  ​#:co-first authors, *: co-corresponding authors doi:10.1021/acs.nanolett.9b03364

• Shumin Xia, Bena Lim, Yilin Wang, Zhen Zhang, Shan Zhang, Chwee Teck Lim, Evelyn K. F. Yim, Pakorn Kanchanawong, “Nanoscale Architecture of the Cortical Actin Cytoskeletons in Embryonic Stem Cells” Cell Reports 28(5), P1251-1267.E7 (2019) doi:10.1016/j.celrep.2019.06.089

• Nisha Bte Mohd Rafiq#, Yukako Nishimura#, Sergey V Plotnikov, Visalatchi Thiagarajan, Zhen Zhang, Shidong Shi, Meenubharathi Natarajan, Virgile Viasnoff, Pakorn Kanchanawong*, Gareth Jones*, and Alexander D. Bershadsky* "A mechano-signalling network linking microtubules, myosin-IIA filaments and integrin-based adhesions" Nature Materials 18, 638-649 (2019). doi:10.1038/s41563-019-0371-y

       #:co-first authors, *: co-corresponding authors

• Yun-Chi Tsai, Wei-Chun Tang, Christine Siok Lan Low, Yen-Ting Liu, Jyun-Sian Wu, Po-Yi Lee, Pakorn Kanchanawong, Liang Gao, and Bi-Chang Chen “Rapid High Resolution 3D Imaging of Expanded Biological Specimens with Lattice Light Sheet Microscopy”  Methods doi: 10.1016/ j.ymeth.2019. 04.006

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• Shumin Xia, Evelyn K. F. Yim, Pakorn Kanchanawong, “Molecular Organization of Integrin-based Adhesion Complexes in mouse Embryonic Stem Cells”, ACS Biomaterials Sciences and Engineering 5(8), 3828-3842 (2019). doi:10.1021/acsbiomaterials.8b01124

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• Samuel F. H. Barnett, Pakorn Kanchanawong. “Visualizing the backbone of focal adhesions” Emerging Topics in Life Sciences 2 (5), 677-680 (2018) doi.org/10.1042/ETLS20180167

• Satoko Sakamoto, Dean Thumkeo, Hiroshi Ohta, Zhen Zhang, Pakorn Kanchanawong, Takayoshi Fu, Sadanori Watanabe, Yoshitaka Fujiwara, Masahito Ikawa, Naoki Watanabe, Mitinori Saitou and Shuh Narumiya  “mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility” PLOS Biology 2018; 9: e2004874 doi.org: 10.1371/journal.pbio.2004874

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• Ravikrishna Ramanujam, Ziyin Han, Zhen Zhang, Pakorn Kanchanawong, and Fumio Motegi. “Establishment of the PAR-1 cortical gradient by the aPKC-PRBH circuit.” Nature Chemical Biology 2018; 14:917-927 doi.org/10.1038/s41589-018-0117-1

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• Chwee Tat Koe, Ye Sing Tan, Max Lönnfors, Seong Kwon Hur, Christine Siok Lan Low, Yingjie Zhang, Pakorn Kanchanawong, Vytas A. Bankaitis, Hongyan Wang. “Vibrator and PI4KIIIa Govern Neuroblast Polarity by Anchoring Non-muscle Myosin II” eLife 2018; 7:e33555 doi: 10.7554/eLife.33555

• Zhen Zhang, Yen Wei Lim, Peng Zhao, Pakorn Kanchanawong*, Fumio Motegi*. “ImaEdge: A quantitative image analysis platform for cortical protein dynamics during cell polarization.” Journal of Cell Science 2017: 130(24), 4200-4212 doi: 10.1242/jcs.206870  * co-corresponding authors

• Rupprecht JF, Martinez-Marrades A, Changede R, Zhang Z, Kanchanawong P, Tessier G. "Trade-offs between spatial and temporal resolutions in stochastic super-resolution microscopy techniques" Optics Express 25(19), 23146-23163 (2017). doi:10.1364/OE.25.023146

• Shumin Xia, Pakorn Kanchanawong. "Nanoscale Mechanobiology of Cell Adhesions" Seminars in Cell and Developmental Biology (2017) doi:10.1016/j.semcdb.2017.07.029

• Zhen Zhang, Shumin Xia, Pakorn Kanchanawong. “An Integrated Enhancement and Reconstruction Strategy for the Quantitative Extraction of Actin Stress Fibers from Fluorescence Micrographs” BMC Bioinformatics (2017) 18:268. doi: 10.1186/s12859-017-1684-y.

• Kapish Gupta, Qiushi Li, Jun Jun Fan, Eliza Li Shan Fong, Ziwei Song, Shupei Mo, Haoyu Tang, Inn Chuan Ng, Chan Way Ng, Pornteera Pawijit, Shuangmu Zhuo, Chen-Yuan Dong, Boon Chuan Low, Aileen Wee, Yock Young Dan, Pakorn Kanchanawong, Peter So, Virgile Viasnoff, Hanry Yu. "Actomyosin Contractility Drives Bile Regurgitation as an Early Response During Obstructive Cholestasis" Journal of Hepatology, 66(6), 1231-1240, 2017.  doi:10.1016/j.jhep.2017.01.026

• Cristina Bertocchi, Yilin Wang, Andrea Ravasio, Yusuke Hara, Yao Wu, Talgat Sailov, Michelle A. Baird, Michael W. Davidson, Ronen Zaidel-Bar, Yusuke Toyama, Benoit Ladoux, Rene-Marc Mege, and Pakorn Kanchanawong. “Nanoscale architecture of cadherin-based cell adhesions” Nature Cell Biology 19 (1), 28-37, 2017. doi:10.1038/ncb3456              *  News and Views in Nature Cell Biology

• Yeo ELL, Joshua U, Cheah J, Neo DJH, Goh WI, Kanchanawong P, Soo KC, Thong PSP, Kah JCY: "Exploiting the protein corona around gold nanorods for low-dose combined photothermal and photodynamic therapy." Journal of Materials Chemistry B, 5 (2), 254-268, 2017. doi: 10.1039/C6TB02743A 

• Z Zhang, Y Nishimura, P Kanchanawong "Extracting Microtubule Networks from Superresolution Single-Molecule Localization Microscopy Data" Molecular Biology of the Cell, 28 (2), 333-345, 2017. doi:10.1091/mbc.E16-06-0421

• ​Wang, Y., Kanchanawong, P. "Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)." J. Vis. Exp. (118), e54774, doi:10.3791/54774 (2016). doi: 10.3791/54774

• J. Liu, Y. Wang, W. I. Goh, H. Goh, M. A. Baird, S. Ruehland, S. Teo, N. Bate, D. R.   Critchley, M. W. Davidson, P. Kanchanawong, “Talin determines the nanoscale architecture of focal adhesions” PNAS, 112 (35), E4864-E4873. doi: 10.1073/pnas.1512025112

• Y. Wu, P. Kanchanawong, R. Zaidel-Bar, “Actin-Delimited Adhesion-Independent Clustering of E-Cadherin Forms the Nanoscale Building Blocks of Adherens Junctions”   Developmental Cell. (2015)  32 (2), 139-154. doi: 10.1016/j.devcel.2014.12.003

• G. Shtengel, Y. Wang, Z. Zhang, W. I. Goh, H. F. Hess, P. Kanchanawong. “Imaging cellular ultra-structure by PALM, iPALM, and correlative iPALM-EM.” Methods In Cell Biology, Elsevier (Eds. T. Wittman & J. Waters), (2014). doi: 10.1016/B978-0-12-420138-5.00015-X​

• C. Bertocchi, W. I. Goh, Z. Zhang, P. Kanchanawong. “Nanoscale Imaging by Superresolution Fluorescence Microscopy and Its Emerging Applications in Biomedical Research.” Critical Reviews in Biomedical Engineering, 41.4-5 (2013). doi: 10.1615/CritRevBiomedEng.2014010448 

• A. Nomachi, M. Yoshinaga, J. Liu, P. Kanchanawong, D. Thumkeo, T Watanabe, S. Narumiya, T. Hirata. “Moesin Controls Clathrin-Mediated S1PR1 Internalization in T Cells”. Plos ONE. 8(12): e82590, 2013. doi:10.1371/journal.pone.0082590

• P. Kanchanawong, C. M. Waterman. “Localization-based Super-resolution Imaging of Cellular Structures”, Adhesion Protein Protocols, Methods in Molecular Biology, Humana Press, A. Coutts (ed.), 1046:59-84, 2013. doi:10.1007/978-1-62703-538-5_4​

• G. Shtengel, J. Galbraith, C. Galbraith, P. Kanchanawong, C. Waterman, H. Hess. “Multicolor Interferometric Photo-Activation Localization Microscopy with Extended Axial Range”, Microscopy and Microanalysis, 17(S2): 6-7, 2011. doi:10.1017/S1431927611000900

• P. Kanchanawong, C. M. Waterman. “Advances in light-based imaging of three-dimensional cellular ultrastructure”, Current Opinion in Cell Biology, 24:1-9, 2011 doi:10.1016/j.ceb.2011.11.010

• R.S. Fischer, Y. Wu, P. Kanchanawong, H. Shroff, C. M. Waterman. “Microscopy in 3D: a biologist’s toolbox”, Trends in Cell Biology, 21(12): 682-691, 2011. doi:10.1016/j.tcb.2011.09.008

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NIH (2007-11)

• P. Kanchanawong, G. Shtengel, A. Pasapera-Limon, E. Ramko, M.W. Davidson, H.F. Hess, C. M. Waterman. “Nanoscale Architecutre of Integrin-based Adhesions”, Nature, 468(7323): 580-4, 2010.  doi:10.1038/nature09621

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• W.D. Shin, R.S. Fischer, P. Kanchanawong, Y. Kim, J. Lim, K.A. Meyers, Y. Nishimura, S.V. Plotnikov, I. Thievessen, D. Yarar, B. Sabass, C.M. Waterman, A Versatile, Multicolor Total Internal Reflection Fluorescence and Spinning Disk Confocal Microscope System for High-Resolution Live Cell Imaging, in: R.D. Goldman, J.R. Swedlow, D.L. Spector (Eds.), Live Cell Imaging: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2010.

•  G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter and H. F. Hess.  “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure”, PNAS, 106 (9), 3125-3130, 2009. doi: 10.1073/pnas.0813131106

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Stanford (2001-07)

•   L. N. Silverman, P. Kanchanawong, T. P. Treynor, S. G. Boxer. “Stark spectroscopy of mixed valence systems”, Phil. Trans. R. Soc. A, 366, 33-45, 2008. doi: 10.1098/rsta.2007.2137

•  X. Shu, K. Kallio, X. Shi, P. Abbyad, P. Kanchanawong, W. Childs, S. G. Boxer, and S. J. Remington. “Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D. 1. Mutagenesis and Structural Studies”, Biochemistry, 46 (43), 12005 -12013, 2007. doi: 10.1021/bi7009037        

•    X. Shi, Paul Abbyad, X. Shu, K. Kallio, P. Kanchanawong, W. Childs, S. J. Remington, and S. G. Boxer. “Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D. 2. Unusual Photophysical Properties”, Biochemistry, 46 (43), 12014 -12025, 2007.  doi: 10.1021/bi700904a        

•   P. Kanchanawong, M. G. Dahlbom, T. P. Treynor, J. R. Reimers, N.S. Hush and S. G. Boxer, “Charge delocalization in the special pair radical cation of mutant reaction centers of Rhodobacter sphaeroides from Stark spectra and non-adiabatic spectral simulations”, J. Phys. Chem. B. 110 (37), 18688-18702, 2006. doi: 10.1021/jp0623894

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Cornell (1997-2001)

•  G. Andre, P. Kanchanawong, R. Palma, H. Cho, X. Deng, D. Irwin, M. E. Himmel, D. B. Wilson and J. W. Brady, “Computational and experimental studies of the catalytic mechanism of Thermobifida fusca Cellulase Cel6A (E2)”, Protein Engineering. Vol. 16: 125-134, 2003. doi: 10.1093/proeng/gzg017

Co-supervised Graduate Student

Harini Rajendiran (with  Virgile Viasnoff)

Kuo Xuan (with Winston Zhao)

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Undergraduates (& current position)

Chai Jun Hao (FYP 2021)

Eldred Sng (FYP 2021, Duke-NUS)

Jonathan San (FYP 2021)

Ka Hui Yi (FYP 20201)

Cheryl Lee (FYP 2020, Illumina Inc.)

Kallista Wong (Independent Study 2020)

An Honglin (FYP, MSc NUS Mathematics)  

Samantha Chang Shi Min (Research Attachment/FYP 2018-2019, Research Assistant MBI)

Kevin Lim Zi Rong (FYP)

Arulchevan Vijayakumar (FYP2019, Research Assistant MBI)

Julian Siew Tze Kang (FYP)

Elena Poh Woon Li (FYP)

Sim Yi Herng (FYP, Micron Technology)

Wong Kin Sun (FYP, PhD student Biomedical Engineering)

Kwok Zi Jun (Research attachment)

Udpa Namitha Sujit (FYP) 

Ankshita Prasad (FYP, Research Fellow NUS) 

Akshaya Srinivisan (FYP, Research Fellow NUS)

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Former Lab Members (& current position)

April Kyi Thwin (Moderna Inc. USA)

Chen Youchao (Graduate student, University of Warwick, UK)

Xia Shumin, Ph.D. (Wuxi Biologics, China)

Zhang Yuzi (Graduate student, HKUST, Hong Kong SAR)

Azita Gorji, Ph.D. (Postdoctoral Fellow, Institut Curie, Paris, France)

Cristina Bertocchi, Ph. D. (Assistant Professor, Pontificia Universidad Catolica de Chile, Santiago, Chile)

Yukako Nishimura, Ph.D. (Hokkaido University, Japan)

Zhang Zhen, Ph. D. (Alibaba Inc.)

Huang Shuangru, Ph. D. (SpesBio Ltd., Hangzhou, China)

Gary Guo Zhenhuan

Goh Wah Ing, Ph. D. (A*STAR Microscopy Platform

Tian Xingyue (Chengdu, China)

Chaw Su Thwin (Research Associate, Nanyang Technological University)
Teo Shijia (Graduate Student, University of Frieburg, Germany)
Jaron Liu, Ph. D. (Mayo Clinic)

Goh Honzhen (Research Assistant, NUS)

Talgat Sailov (Zeiss Microscopy)

Svenja Ruehland (Ph.D. Student, Ludwig Maximillian University of Munich, Germany)

Ratul Chowdhury (Harvard Medical School)

Eric Li, Ph. D. (Postdoctoral Fellow, University of Sydney, Australia)

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