Nikon Eclipse Ti-E Inverted Microscope

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• Nikon Eclipse Ti -E with Perfect Focus, Motorized TIRF Illuminator, calibrated for VIAFLIC/SAIM Surface-generated structured illumination microscopy

• Omicron fiber-coupled laser combiner: 50 mW 488 nm, 60 mW 561 nm

• OkoLab Temperature, Humidity, CO2 controller for Live cell imaging

• Hamamatsu Orca Flash 4.0 sCMOS camera

• Lumencor SOLA for Epifluorescence excitation

• Cairn OptoLED for Diascopic illumination

• Objective Lenses: 60X NA 1.49 Apo TIRF, 60 X NA1.2 WI, 100X NA 1.45 Plan Apo, 40X NA 1.3 Plan Fluor, 20X NA 0.45 ELWD, 20X NA 0.75

• Nikon NIS-Elements

• Application: Immunofluorescence Microscopy, Interference Reflectance Microscopy (IRM), VIAFLIC/SAIM Superresolution Microscopy, FRET Microscopy, Fluorescence Speckle Microscopy, Live cell DIC/Phase-contrast/epifluorescence microscopy.

• Read about our implementation of VIAFLIC/SAIM (SI Methods) in Liu et al., PNAS 2015

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Also check out the state-of-the-art microscopes at MBI core facilities http://microscopy.mechanobio.info/

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iPALM 3D-Superresolution Microscope  see also iPALM page

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• For theoretical foundations and detailed construction/operation/calibration info, see Shtengel et al PNAS 2009, Shtengel et al Methods. Cell Biol. 2014

• See our JoVE video article on using the iPALM

• Dual-opposed Nikon 60X NA 1.49 Apo TIRF objective lenses

• Custom 3-way Hess multiphase beamsplitter (Rocky Mountain Instrument)

• Custom-machined Invar Chassis and Mountings

• Piezo Controllers: Physik Instrument & Newport (Picomotors)

• Servo Controllers: ThorLabs

• Optomechanical Components: Newport, ThorLabs, & Melles Griot

• Filters and Custom Notch Mirror: Semrock & Reynard Corp.

• 3 EMCCD (Andor Ixon Ultra)

• Coherent Lasers: Cube 405 nm, 100 mW; Sapphire 488 nm , 200 mW; Sapphire 561 nm 200 mW; Obis 642 nm, 100 mW.

• A-A AOTF.

• Control PCs: 4 Dell with Intel i5 processor with LabVIEW 2010 on Windows 7 Enterprise.

• On-site Image Processing Workstations (2): Intel Xeon with 32 threads & 192 GB memory.

• MBI Linux Computing clusters (2): 24-Nodes Xeon Cluster & 256 GB memory and 11-Node PBS cluster

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